Abstract: Induction of Tumor-killing Immune Cells by Glioma Antigen and Superantigen SECHongmei LIU1, Xiaoqun ZHANG2, Yanan LIU1, Zhihong MA1, Qingbo ZHANG1Correspondence to: Qingbo ZHANG, E-mail: zqb1955@yahoo.com1Department of Biology, North China Coal Medical University, Tangshan 063009, China2Department of ICU, Fengnan Hospital of Tangshan, Tangshan 063000, ChinaThis work was supported by a grant from Science and Technology Support Program of Hebei Province (No. 09276418D-18)Abstract Objective: To develop tumor-killing immune cells from peripheral blood lymphocytes using glioma associated Ag(GSA) and superantigen SEC and to explore a new method for immunotherapy. Methods: Lymphocytes were isolated from peripheralblood of healthy people and were then treated with GSA and superantigen SEC in vitro. Phenotypes and cytotoxic activity of the effec-tor cells were measured by flow cytometry and cytotoxic assay. Results: The lymphocytes stimulated by GSA and SEC showed moreproliferative activity than the control groups, with a peak effect at 72 h, and showed an increased proportion of CD8 + T cells. Co-stim-ulated effector cells had higher cytotoxic activity against target cells than the control group ( P < 0.05 ). Conclusion: The effector cellsinduced by GSA and SEC have more rapid proliferation, higher cytotoxicity, and selective cytotoxicity against the tumor cells that ini-tially produced the GSA. This study provides a new way of thinking for the development of immunotherapy treatment.Keywords Lymphocyte; Glioma-associated antigen; Superantigen; Phenotype